The length-weight relationship and condition factor of Mudskipper (Periophthalmus barbarus) from Buguma Creek, Rivers State, Nigeria, was carried out. Sampling was done fortnightly for a period of three months (March-May, 2018). The length-weight regression statistics was done using Microsoft Excel Analysis Tool. The result obtained indicated that the physico-chemical parameters were within the range favourable for the growth of fish, except in dissolved oxygen. The length-weight relationship showed negative allometric growth, with b= 2.01. Moreover, P.barbarus had a low condition factor which ranged between 0.89-1.41 in both male and female fish, which is an indication of the retarded growth of the species in this environment.